Quiz for DNA technology

Q1: Which enzyme is used to cut DNA at specific sites in genetic engineering?

A. Restriction endonuclease B. DNA ligase C. DNA polymerase D. RNA polymerase

Q2: Which of the following is not a cloning vector?

A. Plasmid B. Cosmid C. Ti plasmid D. Ligase

Q3: Which host organism is commonly used in recombinant DNA technology?

A. Escherichia coli B. Saccharomyces C. Mycobacterium D. Lactobacillus

Q4: Which enzyme is used to join DNA fragments?

A. DNA ligase B. Helicase C. Polymerase D. Endonuclease

Q5: The first recombinant DNA was constructed by combining DNA from which organisms?

A. SV40 and lambda phage B. Plasmid and E. coli C. SV40 and E. coli D. Plasmid and salmonella

Q6: The process of making multiple copies of a gene is called:

A. Gene amplification B. Gene expression C. Gene mapping D. Gene therapy

Q7: What is the function of selectable marker in DNA cloning?

A. Identifies transformants B. Cuts DNA C. Amplifies DNA D. Synthesizes RNA

Q8: What does PCR stand for?

A. Polymerase Chain Reaction B. Protein Chain Reaction C. Polypeptide Chain Reaction D. Polymerase Control Reaction

Q9: Taq polymerase used in PCR is obtained from:

A. Thermus aquaticus B. E. coli C. Bacillus subtilis D. Pseudomonas

Q10: Which of the following is used as a vector in plant genetic engineering?

A. Tiplasmid of Agrobacterium B. E. coli C. Bacteriophage D. Fungi

Q11: A gene that helps in the selection of transformants is called:

A. Selectable marker B. Vector gene C. Promoter D. Operator

Q12: Which of these is a natural genetic engineer of plants?

A. Agrobacterium tumefaciens B. E. coli C. Bacillus thuringiensis D. Rhizobium

Q13: The vector used in human genome project is:

A. YAC B. BAC C. Cosmid D. Plasmid

Q14: Which enzyme unwinds DNA helix during replication?

A. Helicase B. Ligase C. Polymerase D. Endonuclease

Q15: Which technique is used to separate DNA fragments by size?

A. Gel electrophoresis B. Centrifugation C. PCR D. Blotting

Q16: Which of the following is used to visualize DNA in gel electrophoresis?

A. Ethidium bromide B. Methylene blue C. Safranin D. Crystal violet

Q17: Plasmids are:

A. Extra-chromosomal DNA in bacteria B. Main DNA of bacteria C. Fungal DNA D. Viral proteins

Q18: A complementary DNA (cDNA) is synthesized from:

A. mRNA B. tRNA C. rRNA D. DNA template

Q19: Which of the following helps DNA fragments enter host cells?

A. Competence B. Virulence C. Mutation D. Pathogenicity

Q20: Golden rice is genetically modified to produce:

A. Vitamin A B. Vitamin B12 C. Iron D. Zinc